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Diagnostic tests


These are the tests most commonly used to diagnose dry eyes syndrome.


Normal tear film is continuously available. Blinking maintains the tear film continuity. However if you keep your eyes open long enough, without blinking, the tear film will start breaking up. Your eye will feel uncomfortable forcing you to blink. In patients with dry eyes the tear film is unstable, and breaks up faster. Therefore the tear break up time in patients who have dry eyes is shorter.

  • Tear Film break-up time (TFBUT or FBUT)

    This measures the interval between the individual’s last complete blink and the break-up of his or her tear film

    1. This simple test involves the use of a slit-lamp, set on a bright light setting with a cobalt blue filter:
    2. Instil fluorescein into the lower fornix.
      Ask the patient to blink several times and then stop.
    3. Measure the time between the last blink and the first appearance of a dark spot on the cornea (formation of a dry area) on the otherwise continuously stained tear film.
      A tear break-up time of less than 10 seconds suggests a dry eye.
  • Non-invasive tear break up time (NIBUT)

    In the F B U T test the presence of fluorescein in the tears may stimulate reflex tearing and may also result in changes to the tear film properties. To overcome these potential limitations, Non Invasive Break up time (NIBUT) methods have been developed. They are called “Non Invasive” because the eye is not touched.

    1. Instruments such as a Keratometer, hand-held Keratoscope or Tearscope are required to measure NIBUT.
    2. A pre-rupture phase that precedes actual break up of the tear film can also be observed with some techniques. This pre-rupture phase is termed Tear Thinning Time (TTT). Measurement is achieved by observing the distortion (TTT) and/or break up (NIBUT) of a keratometer mire (the reflected image of keratometer grid). The clinician focuses and views the crisp mires, and then records the time taken for the mire image to distort (TTT) and/or break up (NIBUT).
    3. NIBUT measurements are longer than fluorescein break up time. NIBUT values of less than 15 seconds are consistent with dry eyes.

    Tear volume and production are components of tear film stability and can be affected by dry eye. One common method for determining tear volume is the Schirmer test.

  • Schirmer test

    1. Instil a drop of local anaesthetic into the eye, (optional).
    2. Prepare a filter paper (5mm x 35mm with folded end).
    3. Gently dry the eye.
    4. Apply the filter paper with the folded end hooked onto the lower lid margin at the junction between the middle and outer third (take care not to touch the cornea).
    5. Tell patient to keep their eye open and blink normally.
    6. Measure the amount of wetting after 5 minutes: 13-15mm wetting rules out a dry eye; 6-10mm is borderline and less than 6mm indicates dry eye.
    7. The filter paper strips can cause reflex tearing and may require the use of anaesthetic agents. However, they still provide a measurable clinical indication of dry eye.
  • Phenol Red Test

    A cotton thread impregnated with phenol red dye is used. Phenol red is pH sensitive and changes from yellow to red when wetted by tears.

    1. The crimped end of a 70mm long thread is placed in the lower conjunctival fornix.
    2. After 15 seconds, the length of the colour change on the thread - indicating the length of the thread wetted by the tears -is measured in millimetres.
    3. Wetting lengths should normally be between 9mm and 20mm. Patients with dry eye have wetting values of less than 9mm.
  • Tear Prism Height Test

    A significant amount of information about tear quantity can be gained simply from observing the heights of the upper and lower tear menisci with the slit-lamp biomicroscope.

    There are two techniques used when measuring the height of tear prisms. The first measures the tear meniscus formed on the lower lid margins to give a useful guide to tear volume. This simple technique employs the slit lamp biomicroscope. Excessive or prolonged use of illumination should be avoided to prevent artificial drying of the tear prism.
    The second technique is to compare the tear prism height with the illuminated slit width by setting the slit horizontally in alignment with the lower lid margin, altering the slit width until it appears to match the height of the tear prism. Heights of less than 0.2mm indicate reduced tear fluid quantity.
    Observation of the meniscus profile is also extremely helpful. A regular tear meniscus is typically observed in a healthy eye while a meniscus with a scalloped edge is often associated with a dry eye.

    Ocular staining serves as an indicator of the health of the ocular surface.

  • Rose Bengal Test (No longer widely used)

    End-organ damage to conjunctival and corneal epithelial cells can be assessed via Rose Bengal ocular surface staining, which identifies areas of devitalised tissue:

    1. Instill a drop of Rose Bengal dye into the inferior fornix of the unanesthetised eye.
    2. Ask the patient to blink twice, to spread the red stain over the conjunctiva and cornea.
    3. The ideal time to measure the presence of staining is approximately 3 to 5 minutes after instilling the drops.
    4. Score the staining using a slit-lamp: a pattern of exposure zone (interpalpebral) corneal and bulbar conjunctival staining is typically seen with aqueous tear deficiency.
  • Lissamine Green

    Lissamine green staining like Rose Bengal, colours any desiccated and dying cells on the ocular surface. However, lissamine green may be better accepted by patients as it lacks the slight burning or stinging sensation typically found with rose Bengal.

  • Fluorescein

    Fluorescein staining penetrates areas of the corneal epithelium and conjunctival epithelium where intercellular junctions are disrupted.

    Hyperosmolarity of the tear film is recognized as an important pathogenetic factor in dry eye syndrome (DES). Tear hyperosmolarity may be regarded as the single feature that characterises the condition of “ocular surface dryness”.
    Hyperosmolarity testing has been hampered in the past by difficulties in tear collection and analytic procedures that required laboratory facilities. Recently, ‘lab-ona- chip’ technology has enabled osmolarity measurement to reach the clinical setting.
    The Tearlab Osmolarity System is a new user-friendly tool that only needs tiny volumes for analysis and determines hyperosmolarity semi-automatically.
    The disposable probe, touched onto the lower tear meniscus at the lid margin, collects a nanolitre sample of tears, which is analysed within seconds to provide the clinician with an osmolarity reading. Normal values lie around 304mOsm/kg while values over 320mOsm/kg indicate dry eye.